Hauptrollen:
Dean Winchester - Jensen Ross Ackles
Sam Winchester - Jared Tristan Padalecki
Robert "Bobby" Singer - Jim Beaver
Castiel "Cas" - Misha Collins (Engel)
John Winchester - Jeffrey Dean Morgan
Joanna Beth "Jo" Harvelle - Alona Tal
Ellen Harvelle - Samantha Ferris
Ash - Chad Lindberg
Ruby - Katie Cassidy (oder Genevieve Padalecki)

Nebenrolle:
Mary Winchester - Samantha Smith (Geist)
Azazel - Fredric Lehne (Der gelbäugige Dämon)
FBI Agent Victor Henricksen - Charles Malik Whitfield
Anna Milton - Julie McNiven (Engel)